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KMID : 0545119930030040224
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 4 p.224 ~ p.231
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Sequence Analysis and Expression of Xylanase Gene (xyn Y) from Alkalophilic Bacillus sp. YC-335
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Park, Young Seo
Yum, Do Young/Kim, Jin Man/Bai, Dong Hoon/Yu, Ju Hyun
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Abstract
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The nucleotide sequence of the xylanase gene (xynY) from alkalophilic Bacillus sp. YC-335 was determined and analyzed. An open reading frame of 1,062 base pairs for xynY gene was observed and encoded for a protein of 354 amino acids with a molecular weight of 38,915. S1 nuclease mapping showed that the transcription initiation sites of the xynY gene were different in Bacillus sp. YC-335 and Escherichia coli HB101 (pYS55). S1 mapping also showed that -10 region of the xynY gene recognized by RNA polymerases of E. coli and Bacillus sp. YC-335 were ´TACAGT´ and ´TATGAT´, respectively. A ribosome binding site sequence with the free energy of -17.0 ㎉/㏖ was observed 9 base pairs upstream from the unusual initiation codon, TTG. The proposed signal sequence consisted of 27 amino acids, 2 of which were basic amino acid residues and 21 were hydrophobic amino acid residues. When the amino acid sequences of xylanases were compared, Bacillus sp. YC-335 xylanase showed more than 50% homology with xylanases from B. pumilus, B. subtilis, and B. circulans.
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